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Use of 1H NMR to measure intracellular metabolite levels during growth and asexual sporulation in Neurospora crassa


In this study, we used 1H-NMR to profile the metabolome of N. crassa in extracts isolated from vegetative hyphae and conidia from cultures grown under conditions of high or low sucrose. We also compared wild-type and a G-protein deletion mutant to determine whether lack of gna-3 causes a significant difference in global metabolite profile as a function of carbon availability.